Introduction to Turku BioImaging key technologies (see the Turku BioImaging Black Book for more information).
Optical In Vivo Imaging
The IVIS Lumina II of Turku BioImaging provides quantitative fluorescent and bioluminescent imaging data from mice or rats in vivo. This is an expandable, sensitive imaging system that is easy to use. The setup includes a highly sensitive CCD camera and an imaging chamber. Full automation is provided and the required analytic software is inbuilt.
Inside the imaging chamber a heated stage is available for animals and anesthesia masks are built in. The CCD camera is thermoelectrically (Peltier system) cooled to -90oC, ensuring a low dark current and very little noise. The camera has high quantum efficiency over the entire visible-to-near-infrared spectrum. The stage is height-adjustable, allowing for a field of view of 5-12.5 cm. LED illumination is provided for reference photography, and a halogen lamp is available for fluorescence imaging. The system has a 10-position excitation filter wheel, and replaceable filter wheels to handle emissions at various wavelengths. All of these refinements are motor-controlled via IVIS Living Image software.
Cells and particles expressing luciferase or other fluorescent molecules can be visualized with the IVIS Lumina II system. Macroscopic in vivo imaging enables (for example) phenotyping of transgenic mice, imaging of growing tumor cells in a living animal, follow-up of treatment responses, visualization of inflammation and infections, and arrival of nanoparticles at designated targets.